کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
24062 | 43493 | 2011 | 6 صفحه PDF | دانلود رایگان |
MicroRNAs (miRNAs) are gaining recognition as essential regulators involved in many biological processes, and they are emerging as therapeutic targets for treating disease. Here, we introduce a method for effective delivery of anti-miRNA oligonucleotides (AMOs) using functionalized gold nanoparticles (AuNPs). To demonstrate the ability of AMOs to silence miRNA, we selected miR-29b, which is known to downregulate myeloid cell leukemia-1 (MCL-1), a factor responsible for promoting cell survival. We first generated AuNPs coated with cargo DNA, which was then coupled to complementary DNA linked to an antisense miR-29b sequence. When the AuNPs were delivered into HeLa cells, MCL-1 protein and mRNA levels were increased significantly. Furthermore, apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) was inhibited, proving that AMOs targeting miR-29b were effectively delivered by our innovative AuNP. In addition, we provided evidence that AuNP could deliver other AMOs against miR-21 into two independent cell lines, KGN and 293T, suggesting that the AuNP conjugates can be versatile for any AMO and cell type.
► Design of anti-miRNA oligonucleotides (AMOs) targeting miR-29b.
► Effective delivery of AMOs by functionalized gold nanoparticles (AuNP).
► AuNP-conjugated AMO-miR29b upregulates MCL-1 by blocking miR-29b.
► AuNP-conjugated AMO-miR29b protects against apoptotic stimulation with TRAIL.
Journal: Journal of Biotechnology - Volume 155, Issue 3, 20 September 2011, Pages 287–292