کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2406380 | 1103078 | 2008 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Construction and evaluation of a chimeric pseudoinfectious virus vaccine to prevent Japanese encephalitis Construction and evaluation of a chimeric pseudoinfectious virus vaccine to prevent Japanese encephalitis](/preview/png/2406380.png)
SummaryMultiple vaccines exist to control Japanese encephalitis (JE), but all suffer from problems. We have developed a new type of flavivirus vaccine, a pseudoinfectious virus (RepliVAX WN) that prevents West Nile virus (WNV)-induced disease. Here, we describe production of a chimeric RepliVAX (RepliVAX JE) that expresses the JE virus (JEV) prM and E proteins. Our prototype RepliVAX JE replicated poorly in cells, but blind passage produced a better-growing derivative, and analyses of this derivative allowed us to engineer a second-generation RepliVAX (RepliVAX JE.2) that grew to high titers. RepliVAX JE.2 elicited neutralizing antibodies in both mice and hamsters and provided 100% protection from a lethal challenge with JEV or WNV, respectively. These results demonstrate the utility our RepliVAX platform for producing a JE vaccine.
Journal: Vaccine - Volume 26, Issue 22, 23 May 2008, Pages 2772–2781