کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2407130 1103112 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemokine receptor-mediated delivery of mycobacterial MPT51 protein efficiently induces antigen-specific T-cell responses
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Chemokine receptor-mediated delivery of mycobacterial MPT51 protein efficiently induces antigen-specific T-cell responses
چکیده انگلیسی

Here we evaluated the effects of immunization with a DNA vaccine encoding a fusion protein consisting of macrophage inflammatory protein-1α (MIP-1α) and MPT51 (a major secreted protein from Mycobacterium tuberculosis) on induction of specific CD8+ T cells. The DNA vaccine encoding the fusion protein could induce significantly higher number of the antigen-specific CD8+ T cells in mice than DNA vaccine encoding MPT51 alone. Also, splenocytes from mice immunized with the fusion DNA vaccine expressed higher level of IFN-γ mRNA and protein upon stimulation with an epitope peptide derived from MPT51 than those from mice immunized with a mixture of two DNA vaccines encoding either MPT51 or MIP-1α. These results suggest that DNA vaccine encoding MIP-1α-antigen fusion protein is able to be efficiently internalized into antigen-presenting cells via the chemokine receptor and induce higher level of antigen-specific CD8+ T-cell responses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 26, Issue 40, 19 September 2008, Pages 5165–5169
نویسندگان
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