کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2408476 1103175 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fusion of antigen to Fas-ligand in a DNA vaccine enhances immunogenicity
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Fusion of antigen to Fas-ligand in a DNA vaccine enhances immunogenicity
چکیده انگلیسی

DNA vaccines have considerable potential for the prophylaxis and therapy of a range of diseases, but their potential has not been realised largely due to poor immunogenicity. Fas ligand is a pro-apoptotic molecule, able to induce death of Fas expressing cells. We describe the construction of a DNA vaccine encoding a chimeric fusion between Fas ligand and a truncated version of HIV gp120 as a model antigen. The fusion DNA was used as a priming vaccine, along with boosting with recombinant gp120 protein. Priming with fusion protein DNA resulted in a powerful enhancement of immune responses to the protein boost, and, in the presence of aluminum phosphate, to a strong enhancement in T helper 2 type responses. Fas ligand delivered in a separate plasmid also had an adjuvant effect, although it was weaker than that delivered by the fusion protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 25, Issue 12, 8 March 2007, Pages 2306–2315
نویسندگان
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