کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2408829 1103192 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Germination-independent induction of cellular immune response by Bacillus subtilis spores displaying the C fragment of the tetanus toxin
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Germination-independent induction of cellular immune response by Bacillus subtilis spores displaying the C fragment of the tetanus toxin
چکیده انگلیسی

Bacillus subtilis spores displaying the tetanus toxin fragment C (TTFC) on their surface have been previously shown to induce the production of specific IgG and secretory IgA in mice immunized through the oral or nasal route. Aim of this study was to analyze whether these spores were also able to induce cellular immunity, and whether such immune response was dependent on spore germination in the animal gastro-intestinal tract (GIT). We first developed a germination defective strain of B. subtilis unable to produce viable cells inside the mouse GIT. Germination-defective and congenic wild-type spores both expressing TTFC on their surface were then used to orally immunize Balb/C mice. Both types of spores induced spleen and mesenteric lymph nodes cell proliferation as well as production of IFNγ but not of IL-4 and IL-10 in both districts. Our results indicate that recombinant spores preferentially induce a strong cell-mediated immune response with a Th1 phenotype, independently from their ability to germinate in the GIT.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 25, Issue 5, 15 January 2007, Pages 788–793
نویسندگان
, , , , , , ,