کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2409457 | 1103220 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A non-invasive QPCR method monitoring DNA based therapy of bladder cancer patients
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A non-invasive QPCR method monitoring DNA based therapy of bladder cancer patients A non-invasive QPCR method monitoring DNA based therapy of bladder cancer patients](/preview/png/2409457.png)
چکیده انگلیسی
Real-time PCR technology is highly advantageous for gene studies based on the genetic nature of the transferred material. Urine and blood samples were collected before and after treatment. Treatment of bladder carcinoma patients with plasmid constructs expressing the diphtheria toxin gene was monitored. Detection range from 5 × 106 copies to ≤10 copies was observed with repeatability and reproducibility. No trace amount of the plasmid was detected in the patient's bloodstream, and a continuous clearance in urine, as well, was observed 72 h post-injection. These results show the possibility to design a follow-up method to monitor quantitatively the amount of the plasmid DNA in bladder cancer patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 24, Issue 17, 24 April 2006, Pages 3420–3425
Journal: Vaccine - Volume 24, Issue 17, 24 April 2006, Pages 3420–3425
نویسندگان
Suhail Ayesh, Rasha Abu-Lail, Abraham Hochberg,