کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2409475 1103220 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HIV-1 Tat-coated nanoparticles result in enhanced humoral immune responses and neutralizing antibodies compared to alum adjuvant
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
HIV-1 Tat-coated nanoparticles result in enhanced humoral immune responses and neutralizing antibodies compared to alum adjuvant
چکیده انگلیسی

HIV-1 Tat has been identified as an attractive target for vaccine development and is currently under investigation in clinical trials as both a therapeutic and preventative vaccine for HIV-1. It is well known that protein based vaccines produce poor immune responses by themselves and therefore require adjuvants to enhance immune responses. We have previously reported on the use of anionic nanoparticles (NPs) for enhancing cellular and humoral immune responses to Tat (1–72). The purpose of this study was to further evaluate the immune response of HIV-1 Tat (1–72) coated on anionic nanoparticles compared to alum using various doses of Tat (1–72). Nanoparticles were effective at generating comparable antibody titers at both 1 and 5 μg doses of Tat (1–72), whereas the antibody titers significantly decreased at the lower dose of Tat (1–72) using alum. Anti-sera from Tat (1–72) immunized mice reacted greatest to the N-terminal and basic regions of Tat, with the NP groups showing stronger reactivity to these regions compared to alum. Moreover, the anti-sera from all Tat (1–72) immunized groups contained Tat-neutralizing antibodies and were able to significantly inhibit Tat-mediated long terminal repeat (LTR) transactivation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 24, Issue 17, 24 April 2006, Pages 3564–3573
نویسندگان
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