کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2409556 1103222 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Impact of plasmid stability on oral DNA delivery by Salmonella enterica serovar Typhimurium
چکیده انگلیسی

Live attenuated Salmonellae may overcome limitations with conventional methods of DNA immunisation. This study examined the impact of plasmid stability on oral DNA delivery by the attenuated Salmonella enterica serovar Typhimurium vaccine strain BRD509. A DNA vaccine cassette comprising the C fragment of tetanus toxin under control of the cytomegalovirus (CMV) promoter was ligated into plasmid pcDNA3, pUC18, pBBR122, pACYC184, pRSF1010/CAT, pBR322 and pAT153. In vitro and in vivo stability studies revealed that, with the exception of pcDNA3 and pUC18, the plasmids were retained by BRD509. However, pAT153 was the only plasmid to induce a tetanus toxoid-specific antibody response following oral delivery. Plasmid copy number was found to impact on plasmid stability and the induction of antigen-specific humoral responses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 25, Issue 8, 9 February 2007, Pages 1476–1483
نویسندگان
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