کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2421063 1552483 2007 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro examination of DHA-edible micro algae: 1. Effect on rumen lipolysis and biohydrogenation of linoleic and linolenic acids
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
In vitro examination of DHA-edible micro algae: 1. Effect on rumen lipolysis and biohydrogenation of linoleic and linolenic acids
چکیده انگلیسی

Effects of a docosahexaenoic acid (DHA)-edible algae product, Aquagrow-DHA, on lipolysis and biohydrogenation of linoleic acid (C18:2 n-6) was studied in 24 h in vitro incubations with buffer (20 ml), sheep rumen contents (5 ml), hay (0.4 g) and sunflower oil (20 mg). Four Aquagrow-DHA levels (0, 20.8, 41.6, 83.3 mg) were tested and addition of lard ensured that the total fat content was constant. Increasing Aquagrow-DHA proportionally inhibited hydrogenation of C18:2 n-6, provoking an accumulation of C18:1 trans fatty acids (mainly C18:1 t10 + t11). Analogous incubations for 6 and 24 h with linseed oil (20 mg) instead of sunflower oil, resulted in the accumulation of C18:2 t11c15 and, to a lower extent, C18:1 t10 + t11 and showed that Aquagrow-DHA reduced the rate of lipolysis of linolenic acid (C18:3 n-3). Comparison of these results with incubations with the individual constituents of Aquagrow-DHA and whole algae biomass revealed that the observed effects were related to the presence of DHA. Finally, incubations under a 50:50 H2:CO2 atmosphere suggested a direct inhibitory effect of unesterified DHA on hydrogenating bacteria involved in the conversion of C18:2 t11c15 and C18:1 trans isomers, rather than on H2 availability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Feed Science and Technology - Volume 136, Issues 1–2, 15 July 2007, Pages 63–79
نویسندگان
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