کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2426143 | 1552989 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Gene structure of goose-type lysozyme in the mandarin fish Siniperca chuatsi with analysis on the lytic activity of its recombinant in Escherichia coli Gene structure of goose-type lysozyme in the mandarin fish Siniperca chuatsi with analysis on the lytic activity of its recombinant in Escherichia coli](/preview/png/2426143.png)
A goose-type lysozyme (g-lysozyme) gene has been cloned from the mandarin fish (Siniperca chuatsi), with its recombinant protein expressed in Escherichia coli. From the first transcription initiation site, the mandarin fish g-lysozyme gene extends 1307 nucleotides to the end of the 3′ untranslated region, and it contains 5 exons and 4 introns. The open reading frame of the g-lysozyme transcript has 582 nucleotides which encode a 194 amino acid peptide. The 5′ flanking region of mandarin fish g-lysozyme gene shows several common transcriptional factor binding sites when compared with that from Japanese flounder (Paralichthys olivaceus). The recombinant mandarin fish g-lysozyme was expressed in E. coli by using pET-32a vector, and the purified recombinant g-lysozyme shows lytic activity against Micrococcus lysodeikticus.
Journal: Aquaculture - Volume 252, Issues 2–4, 10 March 2006, Pages 106–113