کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2428937 | 1106462 | 2015 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development of an in vitro model system to study the interactions between Mycobacterium marinum and teleost neutrophils Development of an in vitro model system to study the interactions between Mycobacterium marinum and teleost neutrophils](/preview/png/2428937.png)
• Mycobacterium marinum induced pro-inflammatory cytokine and cytokine-receptor gene expression in neutrophils.
• Neutrophils exhibited chemotaxis toward mycobacteria and internalized high number of bacilli.
• Neutrophils exposed to M. marinum produced reactive oxygen intermediates and killed internalized bacilli.
• M. marinum induced both apoptosis and necrosis of neutrophils.
The lack of a reliable mammalian neutrophil in vitro culture system has restricted our ability to examine their precise roles in mycobacterial infections. Previously, we developed the procedures for the isolation and culture of primary kidney-derived neutrophil-like cells from goldfish that are functionally and morphologically similar to mammalian neutrophils. The cultured primary goldfish neutrophils exhibited prolonged viability and functional effector responses. In this study, we demonstrate that when exposed to live or heat-killed Mycobacterium marinum, goldfish neutrophils increased their mRNA levels for several pro-inflammatory cytokines (il-1β1, il-1β2, tnfα-1, tnfα-2) and the cytokine receptors (ifngr1-1, tnfr1, tnfr2). These neutrophils also exhibited chemotaxis toward live mycobacteria, internalized the bacilli, and produced reactive oxygen intermediates (ROI) in response to pathogen exposure. The survival of intracellular mycobacteria was significantly reduced in activated neutrophils, indicating a robust killing response by these teleost granulocytes. We suggest that this goldfish primary neutrophil in vitro model system will provide important information regarding neutrophil-mediated host defense mechanisms against mycobacteria in teleosts as well as in higher vertebrates.
Journal: Developmental & Comparative Immunology - Volume 53, Issue 2, December 2015, Pages 349–357