Fine mapping and conservation analysis of linear B-cell epitopes of peste des petits ruminants virus nucleoprotein

• Nineteen linear B-cell epitopes were screened out on the NP of PPRV China/Tibet strain.
• Minimal motifs of the nineteen linear B-cell epitopes were identified.
• The minimal motifs were 3–9 amino acids in length.
• Ten of the 19 minimal motifs were fully conserved among 46 PPRV strains.

Nucleoprotein (NP) is the most abundant and highly immunogenic protein of morbillivirus, and is presently the basis of most diagnostic assays for peste des petits ruminants virus (PPRV). In this study, fine epitope mapping and conservation analysis of linear B-cell epitopes on the PPRV NP has been undertaken using biosynthetic peptides. Nineteen linear B-cell epitopes were identified and their corresponding minimal motifs were located on the NP of PPRV China/Tibet/Geg/07-30. Conservation analysis indicated that ten of the 19 minimal motifs were conserved among 46 PPRV strains. Peptides containing the minimal motifs were recognized using anti-PPRV serum from a goat immunized with PPRV vaccine strain Nigeria 75/1. Identified epitopes and their motifs improve our understanding of the antigenic characteristics of PPRV NP and provide a basis for the development of epitope-based diagnostic assays.