کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2468960 | 1555443 | 2008 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth disease virus Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth disease virus](/preview/png/2468960.png)
A novel proximity ligation assay (PLA) using a pan-serotype reactive monoclonal antibody was developed and evaluated for the detection of foot-and-mouth disease virus (FMDV) in clinical samples collected from field cases of disease. The FMDV-specific PLA was found to be 100 times more sensitive for virus detection than the commonly used antigen capture-ELISA (AgELISA). As few as five TCID50 were detected in individual assays, which was comparable with the analytical sensitivity of real-time RT-PCR. Although this assay was capable of detecting diverse isolates from all seven FMDV serotypes, the diagnostic sensitivity of the PLA assay was lower than real-time RT-PCR mainly due to a failure to detect some SAT 1, SAT 2 and SAT 3 FMDV strains. In conclusion, this new PLA format has high analytical sensitivity for the detection of FMDV in clinical samples and may prove valuable as a rapid and simple tool for use in FMD diagnosis.
Journal: Veterinary Microbiology - Volume 127, Issues 3–4, 18 March 2008, Pages 227–236