کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2468967 1555443 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel medium devoid of ruminant peptone for high yield growth of Mycoplasma ovipneumoniae
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
A novel medium devoid of ruminant peptone for high yield growth of Mycoplasma ovipneumoniae
چکیده انگلیسی

Mycoplasma ovipneumoniae is considered an emerging veterinary pathogen causing pneumonia in sheep and goats worldwide. Currently it has not been possible to define a growth medium that yields the maximum growth of M. ovipneumoniae within a short incubation period. Growth yields of M. ovipneumoniae in Eaton's medium are variable and not as consistently high as those seen with other Mycoplasma spp. This study investigated the ability of different M. ovipneumoniae field strains to grow in various media formulations, where PPLO broth was replaced by a vegetable protein source, and comparisons were made in terms of strain viability in Eaton's medium. Studies were also conducted to determine the optimal carbohydrate source for use in the M. ovipneumoniae medium. Generally, it was found that different strains showed good growth in all media tested, with growth yields at 24 h in TSB-1 medium higher than those observed with Eaton's medium. Growth yields reached 108 to 109 cfu ml−1 within 24 h for particular field strains, with all strains achieving this growth level within 48–72 h.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 127, Issues 3–4, 18 March 2008, Pages 309–314
نویسندگان
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