کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2469720 | 1555462 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Secretion of biologically active porcine interleukin-2 by Lactococcus lactis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Secretion of biologically active porcine interleukin-2 by Lactococcus lactis Secretion of biologically active porcine interleukin-2 by Lactococcus lactis](/preview/png/2469720.png)
چکیده انگلیسی
In this study, secretion of two functional recombinant porcine interleukin-2 (rIL-2) proteins by Lactococcus lactis was studied. Two secretion cassettes were constructed in which the secretion was achieved by gene fusion between the lactococcal usp45 secretion signal, a synthetic propeptide and the sequence encoding the mature IL-2. In addition, one of the two secretion cassettes contained the H-domains of L. lactis PrtP. Both of the constructed recombinant IL-2 proteins were found to be secreted in the same quantities, approximately 0.5 mg/l. According to a cell proliferative assay using CTLL-2 cell line the specific biological activities of both purified rIL-2 proteins were found to be of similar levels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 115, Issues 1–3, 15 June 2006, Pages 278–283
Journal: Veterinary Microbiology - Volume 115, Issues 1–3, 15 June 2006, Pages 278–283
نویسندگان
Silja Åvall-Jääskeläinen, Airi Palva,