کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
24810 | 43538 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An analytical method for determining relative specificities for sequential reactions catalyzed by the same enzyme: Application to the hydrolysis of triacylglycerols by lipases
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An analytical method for determining relative specificities for sequential reactions catalyzed by the same enzyme: Application to the hydrolysis of triacylglycerols by lipases An analytical method for determining relative specificities for sequential reactions catalyzed by the same enzyme: Application to the hydrolysis of triacylglycerols by lipases](/preview/png/24810.png)
چکیده انگلیسی
We propose a model for the sequential hydrolysis of ester bonds of triacylglycerols by lipases and use it as the basis for an analytical method for determining the relative specificity of the lipase for the various substrates with which it can react, when the substrates occur simultaneously in a single reaction system. We then apply the method to our own data and literature data involving the hydrolysis of triacylglycerols by lipases. Our model is able to fit well to most of the reaction profiles, enabling the estimation of relative specificities. We discuss the limitations and potential applications of our method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 133, Issue 3, 1 February 2008, Pages 343–350
Journal: Journal of Biotechnology - Volume 133, Issue 3, 1 February 2008, Pages 343–350
نویسندگان
David Alexander Mitchell, Jorge A. Rodriguez, Frédéric Carrière, Jacques Baratti, Nadia Krieger,