کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2485492 1114356 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interindividual Differences in Placental Expression of the SLC22A2 (OCT2) Gene: Relationship to Epigenetic variations in the 5′-Upstream Regulatory Region
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Interindividual Differences in Placental Expression of the SLC22A2 (OCT2) Gene: Relationship to Epigenetic variations in the 5′-Upstream Regulatory Region
چکیده انگلیسی

Organic cation transporters (OCTs) mediate the transport of organic cations and some drugs (e.g., metformin and cimetidine). OCT1, OCT2, and OCT3 are located in the imprinting cluster of the insulin-like growth factor 2 receptor. It has been reported that OCT1 and OCT3 show a biallelic expression, whereas OCT2 undergoes maternal imprinting in the human placenta; however, a loss of the imprinting of OCT2 has recently been reported in some placental samples. This study investigated whether epigenetic mechanisms are involved in interindividual differences in the placental expression of OCT2. Because OCT2 mRNA levels were higher in biallelic samples than that in monoallelic samples, we compared the DNA methylation and chromatin modifications in the promoter regions. There was no remarkable difference in DNA methylation between the mono allelic samples and biallelic samples. In contrast, histone H3 acetylation (H3Ac) was increased in the biallelic samples. A significant negative correlation was observed between the trimethylation of lysine-9 on histone H3 (H3K9me3) and the OCT2 mRNA levels. Our results suggest that H3Ac plays a role in the allelic expression of OCT2. In addition, H3K9me3 in the OCT2 promoter may explain the interindividual differences in placental OCT2 mRNA levels. © 2011 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 100:3875–3883, 2011

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 100, Issue 9, September 2011, Pages 3875–3883
نویسندگان
, , , , ,