کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2486080 1114374 2010 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Methyl trypsin loaded poly(D,L-lactide-coglycolide) nanoparticles for contact lens care*
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Methyl trypsin loaded poly(D,L-lactide-coglycolide) nanoparticles for contact lens care*
چکیده انگلیسی
The need of an enzymatic cleaner for soft contact lens care with an improved ocular safety and stability profile led us to evaluate the use of nanoparticles (NPs) of poly(D,L-lactide-coglycolide) (PLGA) and methyl trypsin (MT). NPs were prepared by double emulsion-solvent evaporation technique. A factorial design was performed to select the lactic acid proportion in the copolymer and conditions of the second sonication. The increment in proportion of lactic acid provided higher particle size results. When the time of second sonication was decreased, the entrapment efficiency (EE) increased. PLGA 50:50 NPs were chosen for further development since PLGA 50:50H NPs settled fast with different particle size in the sediment and PLGA 75:25 NPs led to form aggregates. The addition of glycerol to the NPs provided the highest EE of MT (>90%) while the addition of Tetronic® 1304 promoted the fast release of enzyme initially and decreased the zeta potential (ζ) up to neutral values after gamma irradiation. NPs are expected to be effective as a lens care cleaner after 3 days or even longer with a very low quantity of enzyme released. Formulations showed an acceptable irritation ocular tolerance after in vitro HET-CAM test and in vivo Draize test. © 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 99: 1414-1426, 2010
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 99, Issue 3, March 2010, Pages 1414-1426
نویسندگان
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