کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2486166 | 1114377 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The impact of decreased bead count to determine concentrations of amyloid beta1â42, totalâtau, and phosphorylatedâtau181 in human cerebrospinal fluid using xMAP technology
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
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چکیده انگلیسی
Alzheimer's disease is the leading cause of human dementia. The lack of diagnostic tests and limited therapeutic options has driven the search for endogenous biomarkers. The INNOâBIA AlzBio3 assay is a multiplex flowâbased immunoassay measuring Aβ42, tau, and pâtau in cerebrospinal fluid (CSF). This study assesses assays performance under varying bead count (BC) parameters. Original method validation parameters at 100 BC were acceptable. Reanalyses performed at 3, 10, 25, and 50 BCs were compared to 100 BC data by ANOVA, Bland-Altman analysis, evaluation of concordance correlation coefficients, and frequency distribution of coefficient of variation (CV) ranges. Method validation characteristics were acceptable with 100 BCs. Equivalency for 25 and 50 versus 100 BCs was demonstrated, but not for 3 and 10 BCs. A general trend of decreasing agreement between decreasing BCs and the 100 BC reference resulted in decreases in concordance coefficients Ïc. The frequency of CV values greater than 20% increased with decreasing BCs, and CV values of 5% or less decreased with decreased BCs. Statistical analyses demonstrate that BCs of 3 and 10 are not equivalent with the reference and should not be used as a basis for determination of Aβ42, tau, and pâtau concentration in human CSF. © 2011 WileyâLiss, Inc. and the American Pharmacists Association J Pharm Sci 100:4655-4663, 2011
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 100, Issue 11, November 2011, Pages 4655-4663
Journal: Journal of Pharmaceutical Sciences - Volume 100, Issue 11, November 2011, Pages 4655-4663
نویسندگان
Olaf Bjornstal, Kathryn Rogers, Wei Zhang, Richard Delhaye, Michele Malone, Steve Unger, William Nowatzke,