کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2486254 1114378 2010 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vital microscopic analysis of polymeric micelle extravasation from tumor vessels: Macromolecular delivery according to tumor vascular growth stage
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Vital microscopic analysis of polymeric micelle extravasation from tumor vessels: Macromolecular delivery according to tumor vascular growth stage
چکیده انگلیسی
Particles larger than a specific size have been thought to extravasate from tumor vessels but not from normal vessels. Therefore, various nanoparticles incorporating anticancer drugs have been developed to realize selective drug delivery to solid tumors. However, it is not yet clear whether nanoparticles extravasate readily from all tumor vessels including vessels of microtumors. To answer this question, we synthesized new polymeric micelles labeled with fluorescein isothiocyanate (FITC) and injected them into the tail vein of rats with implanted skinfold transparent chambers. We also analyzed, by means of time-lapse vital microscopy with image analysis, extravasation of FITC micelles from tumor vessels at different stages of growth of Yoshida ascites sarcoma LY80. Polymeric micelles readily leaked from vessels at the interface between normal and tumor tissues and those at the interface between tumor tissues and necrotic areas. The micelles showed negligible extravasation, however, from the vascular network of microtumors less than 1 mm in diameter and did not accumulate in the microtumor. Our results suggest that we must develop a novel therapeutic strategy that can deliver sufficient nanomedicine to microtumors. © 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 99:549-562, 2010
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 99, Issue 1, January 2010, Pages 549-562
نویسندگان
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