کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2486507 | 1114384 | 2010 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effluxing ABC transporters in human corneal epithelium
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کلمات کلیدی
HCEP-glycoprotein - P-گلیکوپروتئینCorneal epithelium - اپیتلیوم قرنلABC transporters - حمل کننده ABCMultidrug resistance transporters - حمل کننده های مقاوم در برابر چندین رژیمPrimary Cells - سلولهای اولیهcell model - مدل سلولmultidrug resistance-associated proteins - پروتئین های مرتبط با مقاومت چند داروییEfflux pumps - پمپ های خروجیCell culture - کشت سلول
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
ATP-binding cassette (ABC) transporters are able to efflux their substrate drugs from the cells. We compared expression of efflux proteins in normal human corneal epithelial tissue, primary human corneal epithelial cells (HCEpiC), and corneal epithelial cell culture model (HCE model) based on human immortal cell line. Expression of multidrug resistance protein 1 (MDR1), multidrug resistance-associated protein 1-6 (MRP1-6) and breast cancer resistance protein (BCRP) was studied using quantitative RT-PCR, Western blot, and immunohistochemistry. Only MRP1, MRP5, and BCRP were expressed in the freshly excised human corneal epithelial tissue. Expression of MRP1 and MRP5 was localized predominantly in the basal cells of the central cornea and limbus. Functional efflux activity was shown in the cell models, but they showed over-expression of most efflux transporters compared to that of normal corneal epithelium. In conclusion, MRP1, MRP5, and BCRP are expressed in the corneal epithelium, but MDR1, MRP2, MRP3, MRP4, and MRP6 are not significantly expressed. HCE cell model and commercially available primary cells deviate from this expression profile. © 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 99:1087-1098, 2010
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 99, Issue 2, February 2010, Pages 1087-1098
Journal: Journal of Pharmaceutical Sciences - Volume 99, Issue 2, February 2010, Pages 1087-1098
نویسندگان
Kati-Sisko Vellonen, Eliisa Mannermaa, Helen Turner, Marika Häkli, J. Mario Wolosin, Timo Tervo, Paavo Honkakoski, Arto Urtti,