کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2487230 | 1114409 | 2009 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Controlled release of Repifermin® from polyelectrolyte complexes stimulates endothelial cell proliferation
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
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![عکس صفحه اول مقاله: Controlled release of Repifermin® from polyelectrolyte complexes stimulates endothelial cell proliferation Controlled release of Repifermin® from polyelectrolyte complexes stimulates endothelial cell proliferation](/preview/png/2487230.png)
چکیده انگلیسی
The therapeutic value of many growth factors is often hindered by the narrow therapeutic index and sustained concentrations required for efficacy. Controlled release approaches provide a valuable tool to achieve these goals; however, growth factor stability must be maintained. Repifermin® is a truncated form of fibroblast growth factor-10, also known as keratinocyte growth factor-2, that exhibits promise in wound healing applications; however, controlled release formulation presents a challenge for this labile protein. Taking advantage of the heparin-binding motif of this class of biopharmaceuticals, Repifermin® was effectively stabilized and packaged in polyelectrolyte complexes. In the presence of dextran sulfate, the unfolding temperature of this growth factor was increased by â¼10°C as confirmed by a variety of spectroscopic techniques. Dextran sulfate with bound Repifermin® was then complexed with several polycations (chitosan, poly-L-lysine, and polyethylenimine) resulting in the formation of â¼250 nm polyelectrolyte complexes that entrapped the protein with â¼70-80% efficiency. Release was controlled for more than 10 days and the mitogenic activity of Repifermin® on human umbilical cord vascular endothelial cells was significantly enhanced, whereas no effect was noted for free Repifermin®. © 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 98:268-280, 2009
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 98, Issue 1, January 2009, Pages 268-280
Journal: Journal of Pharmaceutical Sciences - Volume 98, Issue 1, January 2009, Pages 268-280
نویسندگان
Min Huang, Cory Berkland,