کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2488027 | 1114450 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Near-infrared analysis of protein secondary structure in aqueous solutions and freeze-dried solids
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Near-infrared spectroscopy (NIR) of various proteins (bovine serum albumin, lysozyme, ovalbumin, γ-globulin, β-lactoglobulin, myoglobin, cytochrome-c) was investigated as a possible analytical method of the protein secondary structure in various physical states. The spectra of proteins in aqueous solutions (transmission mode, solvent-compensated) and those in freeze-dried solids (nondestructive diffuse reflection mode) showed several bands at similar frequencies in the combination (4000-5000 cmâ1) and first overtone (5600-6600 cmâ1) spectral regions. The normalized second-derivative near-infrared spectra of proteins in aqueous solutions suggested that some bands indicated α-helix (4090, 4365-4370, 4615, and 5755 cmâ1) and β-sheet (4060, 4405, 4525-4540, 4865, and 5915-5925 cmâ1) structures. The proteins mostly maintained spectra characteristic of their native structure after freeze-drying, although some reductions in α-helical structure and increase in unordered or β-sheet structures were observed. The near-infrared analysis also showed β-sheet formation of heat-treated BSA in aqueous solutions and in subsequently freeze-dried solids. The present results thus indicated that the nondestructive near-infrared analysis can be used for the investigation of dehydration-induced changes in protein secondary structures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 95, Issue 4, April 2006, Pages 781-789
Journal: Journal of Pharmaceutical Sciences - Volume 95, Issue 4, April 2006, Pages 781-789
نویسندگان
Ken-ichi Izutsu, Yasuto Fujimaki, Akiko Kuwabara, Yukio Hiyama, Chikako Yomota, Nobuo Aoyagi,