کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
24924 | 43546 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
We investigated the direct constitution of membrane proteins into giant liposomes in cell-free (in vitro) protein synthesis. Giant liposomes were present in a translation reaction cocktail of a wheat germ cell-free protein translation system. Apo cytochrome b5 (b5) and its fusion proteins were synthesized and directly localized in the liposomes. After the translation reaction, the proteo-liposomes were isolated by simplified discontinuous density-gradient centrifugation. Apo cytochrome b5 conjugated dihydrofolate reductase (DHFR) was synthesized in the same procedure and the protein was directly displayed on the liposome surface. b5 acts as a “hydrophobic tag” for recruitment to the liposome surface.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 133, Issue 2, 20 January 2008, Pages 190–195
Journal: Journal of Biotechnology - Volume 133, Issue 2, 20 January 2008, Pages 190–195
نویسندگان
Shin-ichiro M. Nomura, Satoshi Kondoh, Wakiko Asayama, Akikazu Asada, Shigemichi. Nishikawa, Kazunari Akiyoshi,