کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
24924 43546 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Direct preparation of giant proteo-liposomes by in vitro membrane protein synthesis
چکیده انگلیسی

We investigated the direct constitution of membrane proteins into giant liposomes in cell-free (in vitro) protein synthesis. Giant liposomes were present in a translation reaction cocktail of a wheat germ cell-free protein translation system. Apo cytochrome b5 (b5) and its fusion proteins were synthesized and directly localized in the liposomes. After the translation reaction, the proteo-liposomes were isolated by simplified discontinuous density-gradient centrifugation. Apo cytochrome b5 conjugated dihydrofolate reductase (DHFR) was synthesized in the same procedure and the protein was directly displayed on the liposome surface. b5 acts as a “hydrophobic tag” for recruitment to the liposome surface.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 133, Issue 2, 20 January 2008, Pages 190–195
نویسندگان
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