کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2504055 | 1557450 | 2010 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Brush-shaped polycation with poly(ethylenimine)-b-poly(ethylene glycol) side chains as highly efficient gene delivery vector Brush-shaped polycation with poly(ethylenimine)-b-poly(ethylene glycol) side chains as highly efficient gene delivery vector](/preview/png/2504055.png)
A brush-shaped polymer PHEMA-g-(PEI-b-PEG) with poly(2-hydroxyethyl methacrylate) (PHEMA) backbone and linear poly(ethylenimine)-b-poly(ethylene glycol) (PEI-b-PEG) side chains was synthesized and evaluated as a vector for potential cancer gene therapy. The characterizations by 1H NMR and laser light scattering demonstrated the brush structure of the polymer. PHEMA-g-(PEI-b-PEG) was much less cytotoxic when compared with branched poly(ethylenimine) with Mw of 25 kDa. The capacity of plasmid DNA condensation by PHEMA-g-(PEI-b-PEG) was demonstrated by gel retardation assay, and they formed nanosized complexes with surface zeta potential around 20 mV at N/P ratios higher than 5:1. The complexes of PHEMA-g-(PEI-b-PEG) with plasmid DNA were more efficiently internalized by BT474 cells in comparison with the complexes of PEI25K, leading to higher gene transfection in cells. Further investigation using complexes of PHEMA-g-(PEI-b-PEG) with plasmid DNA encoding wild-type p53 gene showed its potential as a carrier for cancer gene therapy. The complexes of PHEMA-g-(PEI-b-PEG) successfully induced elevated wild-type p53 expression in BT474 cells and led to enhanced apoptosis of BT474 cells. Transfection of wild-type p53 using the complexes also significantly increased the sensitivity of BT474 cells to doxorubicin chemotherapy, suggesting the potential of this carrier in cancer gene therapy.
Journal: International Journal of Pharmaceutics - Volume 392, Issues 1–2, 15 June 2010, Pages 118–126