کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2504142 1557448 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Passive asymmetric transport of hesperetin across isolated rabbit cornea
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
پیش نمایش صفحه اول مقاله
Passive asymmetric transport of hesperetin across isolated rabbit cornea
چکیده انگلیسی

Hesperetin, an aglycone of the flavanone hesperidin, is a potential candidate for the treatment of diabetic retinopathy and macular edema. The purpose of this investigation was to determine solubility, stability and in vitro permeability characteristics of hesperetin across excised rabbit corneas. Aqueous and pH dependent solubility was determined using standard shake flask method. Solution stability was evaluated as a function of pH (1.2–9) and temperature (25 and 40 °C). Permeability of hesperetin was determined across the isolated rabbit cornea utilizing a side-bi-side diffusion apparatus, in the apical to basolateral (A–B) and basolateral to apical (B–A) directions. Hesperetin displayed asymmetrical transcorneal transport with a 2.3-fold higher apparent permeability in the B–A direction compared to the A–B direction. The transport process was observed to be pH dependent. Surprisingly, however, the involvement of efflux transporters or proton-coupled carrier-systems was not evident in this asymmetric transcorneal diffusion process. The passive and pH dependent corneal transport of hesperetin could probably be attributable to corneal ultrastructure, physicochemical characteristics of hesperetin and the role of transport buffer components.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 394, Issues 1–2, 15 July 2010, Pages 60–67
نویسندگان
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