کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2505218 | 1557483 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Evaluation of generations 2, 3 and 4 arginine modified PAMAM dendrimers for gene delivery Evaluation of generations 2, 3 and 4 arginine modified PAMAM dendrimers for gene delivery](/preview/png/2505218.png)
It is a matter of concern to develop and design synthetic non-viral gene carriers with high transfection efficiency and low cytotoxicity in gene therapy. Recently, various arginine conjugated dendrimers showed better performance in transfection and greater viability than polyethyleneimine (PEI). In this study, we synthesized and investigated e-PAM-R G2, 3 and 4 which are biodegradable polyamidoamine (PAMAM) dendrimers modified with arginine and compared that with PAMAM-R series containing amide bonds for gene carriers. For plasmid DNA delivery, the transfection efficiency of e-PAM-R G4 was higher than G3 and G2 and similar to PAMAM-R G4 with favorable cell viability. Moreover, they indicated significantly higher suppression of TEL/AML1 protein, maybe due to rapid olidonucleotide (ODNs) release through biodegradability of e-PAM-R. These results suggest that biodegradable and non-toxic e-PAM-R may be useful carriers for the gene including plasmid DNA, antisense ODNs and si-RNA.
Journal: International Journal of Pharmaceutics - Volume 363, Issues 1–2, 3 November 2008, Pages 199–205