کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2511614 | 1118025 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA](/preview/png/2511614.png)
Yellow head virus (YHV) is an invertebrate nidovirus which causes a severe mortality in cultured Penaeus monodon. The mortality may be prevented by prior treatment of shrimps with YHV-protease dsRNA. Whether the YHV infected shrimp might be cured by the dsRNA remains to be investigated. P. monodon injected with 10−6 YHV showed a high virus replication and mortality within 2 days. Injection of 25 μg YHV-protease dsRNA at 3, 6, 12 or 24 h post YHV infection showed a strong inhibition of YHV replication up to 12 h. Unrelated dsRNA-GFP showed no inhibition, indicating that the inhibition was nucleic acid sequence specific through RNAi pathway. Shrimp mortality could be prevented at 3 h post YHV infection by the dsRNA, but not at 24 h. These results demonstrate that YHV-protease dsRNA gives therapeutic effect and pave the way to develop a cure for YHV-infected shrimps.
Journal: Antiviral Research - Volume 74, Issue 2, May 2007, Pages 150–155