کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2512928 1118384 2012 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hsp90 inhibitors sensitise human colon cancer cells to topoisomerase I poisons by depletion of key anti-apoptotic and cell cycle checkpoint proteins
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Hsp90 inhibitors sensitise human colon cancer cells to topoisomerase I poisons by depletion of key anti-apoptotic and cell cycle checkpoint proteins
چکیده انگلیسی

Hsp90 and topoisomerase I are both targets for chemotherapeutic agents. Topoisomerase I poisons are standard clinical treatments, whilst Hsp90 inhibitors are progressing through clinical trials. We have demonstrated that when an Hsp90 inhibitor and topoisomerase I poison are combined they produce a synergistic increase in apoptosis in both p53+/+ and p53−/− HCT116 human colon cancer cells. Lack of p53 is associated with an increase in sensitivity to the combination treatment; p53+/+ cells treated with the topoisomerase I poison topotecan (TPT) arrest at G2, whereas in p53−/− cells the additional presence of the Hsp90 inhibitor geldanamycin (GA) selectively abrogates the G2M checkpoint. More importantly we report that there is a common underlying p53-independent mechanism behind the observed synergistic combined drug effect. We show that concurrent treatment with GA and TPT is able to reverse TPT induced up-regulation of the anti-apoptotic protein Bcl2 in both p53+/+ and p53−/− HCT116 cells. The data suggests that inhibition of Hsp90 mediates down-regulation of Bcl2 following the combination treatment and cause a synergistic increase in apoptosis in both p53+/+ and p53−/− HCT116 cells; p53−/− HCT116 cells are more sensitive to the treatment because they also fail to arrest at G2 in the cell cycle.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 83, Issue 3, 1 February 2012, Pages 355–367
نویسندگان
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