کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2524353 1119559 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HDAC inhibitor M344 suppresses MCF-7 breast cancer cell proliferation
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
HDAC inhibitor M344 suppresses MCF-7 breast cancer cell proliferation
چکیده انگلیسی

Histone deacetylase (HDAC) inhibitors represent a novel class of drugs that selectively induce cell cycle arrest and apoptosis in transformed cells. This study examined, for the first time, the effects of the relatively new HDAC inhibitor, M344 [4-dimethylamino-N-(6-hydroxycarbamoylhexyl)-benzamide], on the proliferation of MCF-7 breast cancer cells. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays revealed significant concentration- and time-dependent decreases in MCF-7 cell proliferation following treatment with M344 (1-100 μM). In contrast to the significant induction of p21waf1/cip1 mRNA expression following treatment with M344 (10 μM) for 1 or 3 days, there was a significant decrease in p53 mRNA expression, although p53 protein levels were unchanged. Similar treatment with M344 also induced expression of the pro-apoptotic genes, Puma and Bax, together with the morphological features of apoptosis, in MCF-7 cells. The results of this study reinforce previous findings indicating that HDAC inhibitors are an important group of oncostatic drugs, and show that M344 is a potent suppressor of breast cancer cell proliferation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 66, Issue 3, April 2012, Pages 232–236
نویسندگان
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