کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
25428 | 43573 | 2007 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Soluble expression, purification and functional identification of a disulfide-rich conotoxin derived from Conus litteratus Soluble expression, purification and functional identification of a disulfide-rich conotoxin derived from Conus litteratus](/preview/png/25428.png)
Conotoxins are a diverse array of small peptides mostly with multiple disulfide bridges. These peptides become an increasing significant source of neuro-pharmacological probes and drugs as a result of the high selectivity for ion channels and receptors. Usually, the analogue of natural conotoxins is produced by means of chemical synthesis. Here, we present a simple and fast strategy of producing disulfide-rich conotoxins via recombinant expression. By fused with thioredoxin and His tag, a novel O-superfamily conotoxin lt7a was successfully expressed in Escherichia coli and purified, resulting in a high yield of recombinant lt7a about 6 mg/l. The purity of target protein is up to 95% as identified by HPLC results. Whole cell patch-clamp recording revealed that the new conotoxin blocked voltage-sensitive sodium channels in rat dorsal root ganglion neurons, indicating it might be a novel μO-conotoxin.
Journal: Journal of Biotechnology - Volume 128, Issue 1, 30 January 2007, Pages 184–193