کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2555225 1125043 2005 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ethanol induces the production of cytokines via the Ca2+, MAP kinase, HIF-1α, and NF-κB pathway
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
Ethanol induces the production of cytokines via the Ca2+, MAP kinase, HIF-1α, and NF-κB pathway
چکیده انگلیسی

In the present study, we sought to investigate the signal transduction pathways of expression of cytokines in the ethanol-stimulated human mast cell line, HMC-1. Ethanol significantly increased the intracellular calcium level in HMC-1. Ethanol also significantly enhanced IL-6, TNF-α, and TGF-β1 production compared with media control, but did not significantly affect the IL-1β production. After 8 h of stimulation, ethanol increased mRNA and protein expression levels of TNF-α and TGF-β1 in HMC-1. The increased cytokine level was significantly inhibited by BAPTA-AM, PD98059, and SB203580. These inhibitors also inhibited ethanol-induced ERK and p38 MAPK phosphorylation. Ethanol resulted in a great increase in protein levels and promoter activity driving luciferase expression of HIF-1α and NF-κB in HMC-1 cells, but it did not affect on HIF-1α mRNA expression. Our observations show that calcium, MAPK activation, HIF-1α, and NF-κB are necessary for ethanol-induced TNF-α and TGF-β1 expression. These results may have important implications for the study of alcohol-related diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Life Sciences - Volume 77, Issue 17, 9 September 2005, Pages 2179–2192
نویسندگان
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