کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2571105 | 1128617 | 2008 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genome-wide effects of acute progressive feed restriction in liver and white adipose tissue
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کلمات کلیدی
TSSFDRRT-PCRqRT-PCRTFBSSREMicroarraysquantitative RT-PCR - RT-PCR کمیPPRE - ارسالWAT, White adipose tissue - بافت چربی سفید، بافت ادیپوز سفیدtranscription start site - رونویسی شروع سایتtranscription factor binding site - سایت اتصال فاکتور رونویسیSterol response element - عنصر پاسخ استرولEnergy metabolism - متابولیسم انرژیFeed restriction - محدودیت خوراکFood entrainment - نوشیدن غذاWAT - چی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Acute progressive feed restriction (APFR) represents a specific form of caloric restriction in which feed availability is increasingly curtailed over a period of a few days to a few weeks. It is often used for control animals in toxicological and pharmacological studies on compounds causing body weight loss to equalize weight changes between experimental and control groups and thereby, intuitively, to also set their metabolic states to the same phase. However, scientific justification for this procedure is lacking. In the present study, we analyzed by microarrays the impact on hepatic gene expression in rats of two APFR regimens that caused identical diminution of body weight (19%) but differed slightly in duration (4 vs. 10Â days). In addition, white adipose tissue (WAT) was also subjected to the transcriptomic analysis on day-4. The data revealed that the two regimens led to distinct patterns of differentially expressed genes in liver, albeit some major pathways of energy metabolism were similarly affected (particularly fatty acid and amino acid catabolism). The reason for the divergence appeared to be entrainment by the longer APFR protocol of peripheral oscillator genes, which resulted in derailment of circadian rhythms and consequent interaction of altered diurnal fluctuations with metabolic adjustments in gene expression activities. WAT proved to be highly unresponsive to the 4-day APFR as only 17 mRNA levels were influenced by the treatment. This study demonstrates that body weight is a poor proxy of metabolic state and that the customary protocols of feed restriction can lead to rhythm entrainment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 230, Issue 1, 1 July 2008, Pages 41-56
Journal: Toxicology and Applied Pharmacology - Volume 230, Issue 1, 1 July 2008, Pages 41-56
نویسندگان
Raimo Pohjanvirta, Paul C. Boutros, Ivy D. Moffat, Jere Lindén, Dominique Wendelin, Allan B. Okey,