کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
25792 | 43601 | 2006 | 8 صفحه PDF | دانلود رایگان |
To enhance the production of micrococcin GO5, a bacteriocin produced by Micrococcus sp. GO5, cultivation conditions and medium composition were optimized. The optimal initial pH and temperature for bacteriocin production were 7.0–9.0 and 37 °C, respectively. Micrococcus sp. GO5 displayed the highest micrococcin GO5 activity when grown in modified MRS medium that contained lactose or sucrose, rather than glucose, as a carbon source. The maximum bacteriocin activity was obtained in modified MRS medium containing 0.5% tryptone and 1.0% yeast extract as nitrogen sources instead of the other nitrogen sources present in MRS medium. Bacteriocin production was greatly affected by the concentration of K2HPO4; strain GO5 produced eight-fold more bacteriocin in medium containing 2.0–2.5% K2HPO4 than in medium containing 0.2% K2HPO4. The optimal concentration of MgSO4·7H2O for bacteriocin production was 0.5%. The production of micrococcin GO5 was increased 32-fold in shake flask culture and 16-fold in a bioreactor using the optimized medium (TY medium), compared with culturing in MRS medium.
Journal: Journal of Biotechnology - Volume 121, Issue 1, 2 January 2006, Pages 54–61