Hispidin derived from Phellinus linteus affords protection against acrylamide-induced oxidative stress in Caco-2 cells

• Hispidin inhibits acrylamide-induced cell viability decrease in Caco-2 cells.
• Hispidin attenuates acrylamide-mediated ROS increase and MMP decrease.
• Hispidin prevents acrylamide-mediated GSH depletion.

Acrylamide (AA), a well-known toxicant, has attracted numerous attentions for its presumably carcinogenesis, neurotoxicity and cytotoxicity. Oxidative stress was considered to be associated with acrylamide cytotoxicity, but the link between oxidative stress and acrylamide cytotoxicity is still unclear. In the present study, hispidin produced from the edible fungus Phellinus linteus displayed dramatically antioxidant activities against DPPH radicals, ABTS radicals, ferric reducing and hydroxyl radicals, as well as superoxide anion radicals. Moreover, the cytoprotective effect of hispidin against AA-induced oxidative stress was verified upon Caco-2 cells according to evaluate the cell viability, intracellular ROS, mitochondrial membrane potential (MMP) and glutathione (GSH) in the presence or absence of AA (5 mM) in a dose-dependent manner. Collectively, our results demonstrated for the first time that hispidin was able to inhibit AA-induced oxidative stress, which might have implication for the dietary preventive application.