کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2581195 1561648 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dual action of phenylarsine oxide on the glucose transport activity of GLUT1
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Dual action of phenylarsine oxide on the glucose transport activity of GLUT1
چکیده انگلیسی

An early event in the toxic effects of organic arsenic compounds, such as phenylarsine oxide (PAO), is an inhibition of glucose uptake. Glucose uptake involving the glucose transporter, GLUT4 is inhibited by PAO indicating an importance of vicinal sulfhydryls in insulin-stimulated glucose uptake. However, the data on effects of PAO on GLUT1 are conflicting. This study investigated the effects of PAO on glucose uptake in L929 fibroblast cells, cells, which express only GLUT1. The data presented here reveal a dual effect of PAO. At low concentrations or short exposure times PAO stimulated glucose uptake reaching a peak activation of about 400% at 3 μM. At higher concentrations (40 μM), PAO clearly inhibited glucose uptake. At intermediate concentrations (10 μM), PAO had no effect under basal conditions but completely inhibited activation of glucose uptake by glucose deprivation and partially inhibited methylene blue-stimulated glucose uptake. PAO increased the specific binding of cytochalasin B to GLUT1 suggesting a direct interaction with the transporter. These data are most consistent with PAO interacting with multiple proteins that regulate the activity of this transporter, one of which may be GLUT1 itself. The identity of these proteins will require further investigation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 182, Issues 2–3, 10 December 2009, Pages 199–203
نویسندگان
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