کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2581444 1561649 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of abasic sites by the drinking-water mutagen MX in Salmonella TA100
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Induction of abasic sites by the drinking-water mutagen MX in Salmonella TA100
چکیده انگلیسی

Mutagen X (MX) is a chlorinated furanone that accounts for more of the mutagenic activity of drinking water than any other disinfection by-product. It is one of the most potent base-substitution mutagens in the Salmonella (Ames) mutagenicity assay, producing primarily GC to TA mutations in TA100. MX does not produce stable DNA adducts in cellular or acellular DNA. However, theoretical calculations predict that it might induce abasic sites, which it does in supercoiled plasmid DNA but not in rodents. To investigate the ability of MX to induce abasic sites in cellular DNA, we used an aldehydic site assay to detect abasic sites in DNA from Salmonella TA100 cells treated for 1.5 h with MX. At 0, 2.3, and 4.6 μM, MX induced mutant frequencies (revertants/106 survivors) and percent survivals of 2 (100%), 14.9 (111%), and 59.3 (45%), respectively. The frequencies of abasic sites (sites/105 nucleotides) for the control and two concentrations were 5.9, 6.2, and 9.7, respectively, with the frequency at the highest concentration being significant (P < 0.001). These results provide some evidence for the ability of MX to induce abasic sites in cellular DNA. However, the lack of a dose response makes it unclear whether this DNA damage underlies the mutagenic activity of MX.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 180, Issue 3, 14 August 2009, Pages 340–343
نویسندگان
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