کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2591568 | 1131816 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Unrelated developmental neurotoxicants elicit similar transcriptional profiles for effects on neurotrophic factors and their receptors in an in vitro model
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
NTFMicroarraysNGFFGFBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز analysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceTrk - تراکneuronal development - توسعه عصبیOrganochlorine insecticides - حشره کش های آلی کلرOrganophosphate insecticides - حشره کش های ارگانوفسفرهDiazinon - دیازینونDieldrin - دیلرینPC12 cells - سلول های PC12Neurotoxicity - سمیت عصبیMetal neurotoxicity - عصبی فلزیNeurotrophic factors - عوامل نوروترفیکnerve growth factor - فاکتور رشد عصبfibroblast growth factor - فاکتور رشد فیبروبلاستBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزNickel - نیکلTyrosine kinase receptor - گیرنده تیروزین کینازtyrosine kinase receptors - گیرنده های تیروزین کیناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Diverse developmental neurotoxicants can often produce similar functional and behavioral outcomes. We examined an organophosphate pesticide (diazinon), an organochlorine pesticide (dieldrin) and a metal (Ni2+) for effects on the expression of neurotrophic factors and their receptors and modulators in differentiating PC12 cells, an in vitro model of neuronal development. Each agent was introduced at 30 µM for 24 or 72 h, treatments devoid of cytotoxicity. Using microarrays, we examined the mRNAs encoding members of the fibroblast growth factor (fgf) family, the neurotrophins (ntfs), brain-derived neurotrophic factor (bdnf), nerve growth factor (ngf), the wnt and fzd gene families, and the receptors and modulators for each class. All three agents evoked highly concordant patterns of effects on genes encoding the fgf family, whereas the correlations were poor for the group comprising bdnf, ngf and their respective receptors. For wnt, fzd and their receptors/modulators, the relationships between diazinon and dieldrin were highly concordant, whereas the effect of Ni2+ was less similar, albeit still significantly correlated with the others. Our results show that otherwise disparate developmental neurotoxicants converge on common sets of neurotrophic pathways known to control neuronal differentiation, likely contributing to similarities in functional outcomes. Further, cell culture models can provide a useful initial screen to identify members of a given class of compounds that may be greater or lesser risks for developmental neurotoxicity, or to provide an indication of agents in different classes that might produce similar effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurotoxicology and Teratology - Volume 32, Issue 1, JanuaryâFebruary 2010, Pages 42-51
Journal: Neurotoxicology and Teratology - Volume 32, Issue 1, JanuaryâFebruary 2010, Pages 42-51
نویسندگان
Theodore A. Slotkin, Frederic J. Seidler, Fabio Fumagalli,