Persistent developmental toxicity in rat offspring after low dose exposure to a mixture of endocrine disrupting pesticides

There is growing concern of permanent damage to the endocrine and nervous systems after developmental exposure to endocrine disrupting chemicals. In this study the permanent reproductive and neurobehavioral effects of combined exposure to five endocrine disrupting pesticides, epoxiconazole, mancozeb, prochloraz, tebuconazole and procymidone, were examined. Pregnant and lactating rat dams were dosed with a mixture of the five pesticides at three different doses, or with the individual pesticides at one of two doses.Adverse effects were observed in young and adult male offspring from the group exposed to the highest dose of the mixture. These included reduced prostate and epididymis weights, increased testes weights, altered prostate histopathology, increased density of mammary glands, reduced sperm counts, and decreased spatial learning. As no significant effects were seen following single compound exposure at the doses included in the highest mixture dose, these results indicate cumulative adverse effects of the pesticide mixture.

► Developmental exposure to a mixture of endocrine disrupting pesticides.
► Adverse effects on male reproductive organs and behavior.
► Reduced prostate and epididymis weights, increased testes weights and altered histopathology.
► Increased density of mammary glands and lowered sperm counts.
► Mixture effects at dose levels where the single pesticides had no effects.