کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2598482 1562620 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tuning the inflammatory response to silver nanoparticles via quercetin in Caco-2 (co-)cultures as model of the human intestinal mucosa
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Tuning the inflammatory response to silver nanoparticles via quercetin in Caco-2 (co-)cultures as model of the human intestinal mucosa
چکیده انگلیسی


• Ag-NPs lead to inflammatory response in the intestine.
• Ag-NPs reduced Caco-2 cell viability, increased ROS/RNS and lipid peroxidation.
• Ag-NPs decreased secretion of the IL-8 protein and its gene expression.
• Silver ions had only partial contribution in the Ag-NP-induced toxicity.
• Quercetin protected the intestinal cells from the harmful effect of Ag-NPs.

Interaction of nanoparticles with food matrix components may cause unpredictable health complications. Using an improved Caco-2 cell-based in vitro (co-)culture model the potential of quercetin as one of the major food flavonoids to alter the effect of silver nanoparticles (Ag-NPs) <20 nm in the human intestinal mucosa at real life concentrations was investigated. Ag-NPs (15–90 μg/ml) decreased cell viability and reduced thiol groups, induced oxidative/nitrosative stress and lipid peroxidation and led to activity changes of various antioxidant enzymes after 3 h exposure. The contribution of Ag+ ions within the concentrations released from nanoparticles was shown to be less important, compared to Ag-NPs. While leading to inflammatory response in the intestines, Ag-NPs, paradoxically, also showed a potential anti-infammatory effect manifested in down-regulated IL-8 levels. Quercetin, co-administered with Ag-NPs, led to a reduction of cytotoxicity, oxidative stress, and recovered metabolic activity of Caco-2 cells, suggesting the protective effects of this flavonoid against the harmful effect of Ag-NPs. Quercetin not only alleviated the effect of Ag-NPs on the gastrointestinal cells, but also demonstrated a potential to serve as a tool for reversible modulation of intestinal permeability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 253, 24 June 2016, Pages 36–45
نویسندگان
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