Neonicotinoid formaldehyde generators: Possible mechanism of mouse-specific hepatotoxicity/hepatocarcinogenicity of thiamethoxam

Thiamethoxam (TMX), an important insecticide, is hepatotoxic and hepatocarcinogenic in mice but not rats. Studies of Syngenta Central Toxicology Laboratory on species specificity in metabolism established that TMX is a much better substrate for mouse liver microsomal CYPs than the corresponding rat or human enzymes in forming desmethyl-TMX (dm-TMX), which is also hepatotoxic, and clothianidin (CLO), which is not hepatotoxic or hepatocarcinogenic. They proposed that TMX hepatotoxicity/hepatocarcinogencity is due to dm-TMX and a further metabolite desmethyl-CLO (dm-CLO) (structurally analogous to a standard inducible nitric oxide synthase inhibitor) acting synergistically. The present study considers formation of formaldehyde (HCHO) and N-methylol intermediates as an alternative mechanism of TMX hepatotoxicity/hepatocarcinogenicity. Comparison of neonicotinoid metabolism by mouse, rat and human microsomes with NADPH showed two important points. First, TMX and dm-TMX yield more HCHO than any other commercial neonicotinoid. Second, mouse microsomes give much higher conversion than rat or human microsomes. These observations provide an alternative hypothesis of HCHO and N-methylol intermediates from CYP-mediated oxidative oxadiazinane ring cleavage as the bioactivated hepatotoxicants. However, the proposed mono-N-methylol CYP metabolites are not observed, possibly further reacting in situ.

Figure optionsDownload as PowerPoint slideHighlights
► Thiamethoxam (TMX) is hepatotoxic and hepatocarcinogenic in mice but not rats.
► A previous proposed mechanism involves inducible nitric oxide synthase inhibition.
► TMX and desmethyl-TMX with CYPs yield more HCHO than the other neonicotinoids.
► Mouse CYPs are more active than rat or human in converting TMX or dm-TMX to HCHO.
► HCHO or N-methylol generation is an alternative hypothesis for TMX hepatotoxicity.