کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2599959 1133243 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of Δ6-methyltestosterone in a “dietary supplement” and GC–MS/MS investigations on its urinary metabolism
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Detection of Δ6-methyltestosterone in a “dietary supplement” and GC–MS/MS investigations on its urinary metabolism
چکیده انگلیسی

Since a few years more and more products have appeared on the market for dietary supplements containing steroids that had never been marketed as approved drugs, mostly without proper labeling of the contents. Syntheses and few data on pharmacological effects are available dated back mainly to the 1950s or 1960s. Only little knowledge exists about effects and side effects of these steroids in humans.The present study reports the identification of Δ6-methyltestosterone in a product named “Jungle Warfare”, which was obtained from a web-based supplement store.The main urinary metabolites, 17α-hydroxy-17β-methylandrosta-4,6-dien-3-one (Δ6-epimethyl-testosterone), 17α-methyl-5β-androstane-3α,17β-diol (3α,5β-THMT), and 17β-methyl-5β-androstane-3α,17α-diol, as well as the parent compound excreted after a single oral administration were monitored by GC–MS/MS. Δ6-Epimethyltestosterone and 3α,5β-THMT served for long-term detection (still present in the 181–189 h urine). 17α-Methyltestosterone and its 17-epimer were not detected in the urines (LOD 0.3 ng/mL). The highest concentrations were found in the 14–20.5 h urine for Δ6-epimethyltestosterone (600 ng/mL), and 3α,5β-THMT (240 ng/mL) and in the 36–44.5 h urine for 17β-methyl-5β-androstane-3α,17α-diol (7 ng/mL).For reference methyltestosterone and epimethyltestosterone were dehydrogenated with chloranil. The characterization of the products was performed by GC-MS(/MS) and NMR.

Figure optionsDownload as PowerPoint slideResearch highlights▶ Unapproved Δ6-methyltestosterone identified in dietary supplement. ▶ Steroid ingredient was characterized by GC-MS(/MS) and NMR. ▶ Urinary metabolites were identified and monitored. ▶ In doping control 17α-methyl-5β-androstane-3α,17β-diol may be used in screening.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 201, Issue 2, 5 March 2011, Pages 101–104
نویسندگان
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