کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2601352 1562645 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Taurodeoxycholate-induced intestinal injury is modulated by oxidative stress-dependent pre-conditioning like mechanisms
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Taurodeoxycholate-induced intestinal injury is modulated by oxidative stress-dependent pre-conditioning like mechanisms
چکیده انگلیسی

Mechanisms by which hydrophobic bile salts cause tissue changes below their critical micellar concentration (CMC, 1–2 mM) and above (4–8 mM) remain poorly understood. In this study, rat colonic mucosa was exposed to different concentrations of taurodeoxycholate (TDC), t-butyl-hydroperoxide (t-BH) or glutathione ester with or without pre-incubation with 2 mM TDC. Exposure to 2 mM TDC was associated with 10% higher tissue levels of total glutathione (GSH, basal values: 33.7 ± 3.3 nmol/mg prot). With TDC 8 mM, GSH decreased to 16.4 ± 2.3 nmol/mg prot (P < 0.05), oxidized glutathione (GSSG) increased by 60% (P < 0.05), glutathione peroxidase (GSH-Px) and reductase activities were threefold increased, protein carbonyls fourfold increased, protein sulfhydrils decreased by 78%, lactate dehydrogenase (LDH) and GSSG release in the incubation medium were sixfold higher. In 2 mM TDC pre-treated tissues, the subsequent incubation with 8 mM TDC induced a lower loss of tissue GSH, and a lower release of LDH and GSSG. Pre-incubation with 2 mM TDC partly protected against t-BH toxicity, while glutathione ester protected against 8 mM TDC toxicity. In conclusion, TDC exposure causes opposite effects depending on CMC: induction of antioxidant protective systems including glutathione system (pre-conditioning effect) was observed with TDC below CMC, oxidative damages pointing to decreased mucosal detoxification potential with above CMC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 182, Issues 1–3, 10 November 2008, Pages 36–41
نویسندگان
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