کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2602228 1133376 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of the three most commonly used analytical methods for determination of inorganic arsenic and its metabolites in urine
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Evaluation of the three most commonly used analytical methods for determination of inorganic arsenic and its metabolites in urine
چکیده انگلیسی

This work compares the three most common analytical methods for determination of inorganic arsenic and its metabolites in urine: high performance liquid chromatography coupled to either inductively coupled plasma mass spectrometry or atomic fluorescence spectrometry via hydride generation (high performance liquid chromatography-hydride generation-inductively coupled plasma mass spectrometry (HPLC-HG-ICPMS) and HPLC-HG-atomic fluorescence spectrometry (AFS), respectively) and atomic absorption spectrometry coupled to HG (HG-atomic absorption spectrometry (AAS)). This was done with the focus to find alternatives to ICPMS, the investment and running costs of which are rather high. Between-laboratory comparison of HPLC-HG-ICPMS and HPLC-HG-AFS showed good agreement for inorganic arsenic, methylarsonate (MA) and dimethylarsinate (DMA) (R2 = 0.91, R2 = 0.92 and R2 = 0.90, respectively, N = 86). Within-laboratory comparisons of HPLC-HG-AFS, HPLC-HG-ICPMS and HG-AAS showed good agreement for all arsenic species and the sum of inorganic arsenic and its metabolites in urine (HPLC-HG-ICPMS versus HPLC-HG-AFS: R2 = 0.95; HG-AAS versus HPLC-HG-AFS: R2 = 0.95 and HPLC-HG-ICPMS versus HG-AAS: R2 = 0.97; N = 89). HPLC-HG-AFS was found to be a simple, but high quality alternative to HPLC-HG-ICPMS for the speciation and quantification of inorganic arsenic and its metabolites in urine at arsenic concentrations above 10 μg L−1. Because of its considerably lower costs compared to HPLC-HG-ICPMS, it may be a good alternative in laboratories where the high cost of ICPMS is not justified in relation to the intended use of the instrument.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 168, Issue 3, 5 February 2007, Pages 310–318
نویسندگان
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