کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2603923 | 1133848 | 2007 | 7 صفحه PDF | دانلود رایگان |

Berberine, an isoquinoline plant alkaloid, is known to generate a wide variety of biochemical and pharmacological effects. To elucidate the molecular mechanism of berberine-induced antiproliferative activities, the human promonocytic U937 cells were used. Berberine exhibited dose-dependent antiproliferative effects. Morphological evidence of apoptosis, including apoptotic DNA fragmentation, were observed in cells treated with 75 μg ml−1 of berberine for 24 h. Flow cytometry analysis revealed that berberine had no effect on cell cycle profile of U937 cells, however, sub-G0 fraction (apoptotic cell population) was detected. The percentage of sub-G0 fraction of cells treated with 75 μg ml−1 of berberine was 25.3 ± 1.6%. Berberine induces significant changes in mitochondrial membrane potential of U937 cells. The highest tested concentration of berberine decreased the mitochondrial membrane potential to 15.8 ± 2.4% of control. Additionally, berberine-treated cells had an elevated level of ROS production. Activation of caspase-9 and caspase-3 was also detected, with no caspase-8 activation observed. Taken together, the results clearly demonstrate that berberine induces apoptosis of U937 cells through the mitochondrial/caspase-dependent pathway.
Journal: Toxicology in Vitro - Volume 21, Issue 1, February 2007, Pages 25–31