کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2820834 | 1570102 | 2013 | 7 صفحه PDF | دانلود رایگان |

• Removal of alien sequences (adapters, primers) from raw reads improves the quality of results from downstream analyses.
• AlienTrimmer allows detecting and removing multiple alien sequences in both ends of sequence reads.
• AlienTrimmer performs accurately and has fast running time.
Contaminant oligonucleotide sequences such as primers and adapters can occur in both ends of high-throughput sequencing (HTS) reads. AlienTrimmer was developed in order to detect and remove such contaminants. Based on the decomposition of specified alien nucleotide sequences into k-mers, AlienTrimmer is able to determine whether such alien k-mers are occurring in one or in both read ends by using a simple polynomial algorithm. Therefore, AlienTrimmer can process typical HTS single- or paired-end files with millions of reads in several minutes with very low computer resources. Based on the analysis of both simulated and real-case Illumina®, 454™ and Ion Torrent™ read data, we show that AlienTrimmer performs with excellent accuracy and speed in comparison with other trimming tools. The program is freely available at ftp://ftp.pasteur.fr/pub/gensoft/projects/AlienTrimmer/.
Journal: Genomics - Volume 102, Issues 5–6, November–December 2013, Pages 500–506