کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2821092 | 1160921 | 2012 | 9 صفحه PDF | دانلود رایگان |
High-throughput tag-sequencing (Tag-seq) analysis based on the Solexa Genome Analyzer platform was applied to analyze the gene expression profiling of cucumber plant at 5 time points over a 24 h period of waterlogging treatment. Approximately 5.8 million total clean sequence tags per library were obtained with 143013 distinct clean tag sequences. Approximately 23.69%–29.61% of the distinct clean tags were mapped unambiguously to the unigene database, and 53.78%–60.66% of the distinct clean tags were mapped to the cucumber genome database. Analysis of the differentially expressed genes revealed that most of the genes were down-regulated in the waterlogging stages, and the differentially expressed genes mainly linked to carbon metabolism, photosynthesis, reactive oxygen species generation/scavenging, and hormone synthesis/signaling. Finally, quantitative real-time polymerase chain reaction using nine genes independently verified the tag-mapped results. This present study reveals the comprehensive mechanisms of waterlogging-responsive transcription in cucumber.
► Ethylene and auxin were implicated in waterlogging response of cucumber.
► Carbon metabolism was affected by waterlogging.
► ROS generation or scavenging related genes were involved in waterlogging response.
► qRT-PCR verified the tag-mapped results.
Journal: Genomics - Volume 99, Issue 3, March 2012, Pages 160–168