کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2821556 | 1160964 | 2008 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Differential gene expression analysis of iodide-treated rat thyroid follicular cell line PCCl3 Differential gene expression analysis of iodide-treated rat thyroid follicular cell line PCCl3](/preview/png/2821556.png)
The inhibitory effect of supraphysiological iodide concentrations on thyroid hormone synthesis (Wolff–Chaikoff effect) and on thyrocyte proliferation is largely known as iodine autoregulation. However, the molecular mechanisms by which iodide modulates thyroid function remain unclear. In this paper, we analyze the transcriptome profile of the rat follicular cell lineage PCCl3 under untreated and treated conditions with 10− 3 M sodium iodide (NaI). Serial analysis of gene expression (SAGE) revealed 84 transcripts differentially expressed in response to iodide (p ≤ 0.001). We also showed that iodide excess inhibits the expression of essential genes for thyroid differentiation: Tshr, Nis, Tg, and Tpo. Relative expression of 14 of 20 transcripts selected by SAGE was confirmed by real-time PCR. Considering the key role of iodide organification in thyroid physiology, we also observed that both the oxidized form of iodide and iodide per se are responsible for gene expression modulation in response to iodide excess.
Journal: Genomics - Volume 91, Issue 4, April 2008, Pages 356–366