کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2821568 1160965 2009 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Early whole-genome transcriptional response induced by benzo[a]pyrene diol epoxide in a normal human cell line
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Early whole-genome transcriptional response induced by benzo[a]pyrene diol epoxide in a normal human cell line
چکیده انگلیسی

(±)-anti-benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) is a carcinogen causing bulky-adduct DNA damage. In this study, we investigated early transcriptional signatures induced by various concentrations (0.005, 0.05, and 0.5 μM) of this carcinogen in a normal human cell line (FL human amnion epithelial cells) using the whole-genome Affymetrix HG-U133 Set microarray. The numerous identified genes were involved in multiple functions and higher doses of BPDE elicited more robust expression changes. The disturbance of genes involved in cell cycle regulation, growth and apoptosis was correlated with the S and G2/M phase cell cycle arrest and cytotoxic phenotypes induced by different levels of BPDE. Bioinformatic analysis showed that several transcription factors and their related stress signaling pathways might partly account for the transcriptional signature induced by BPDE. Additionally, gene ontology analysis of the microarray data showed down-regulation of transport, cytoskeleton and DNA repair by 0.5 μM BPDE exposure. In conclusion, this genomic analysis helps to understand the mechanism of cellular response to BPDE.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Genomics - Volume 93, Issue 4, April 2009, Pages 332–342
نویسندگان
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