کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2821631 | 1160976 | 2007 | 11 صفحه PDF | دانلود رایگان |
In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) (OsHK1–4, OsHKL1), 5 HPs (histidine phosphotransfer proteins) (OsHP1–5), 15 type-A RRs (response regulators) (OsRR1–15), 7 type B RR genes (OsRR16–22), and 5 predicted pseudo-response regulators (OsPRR1–5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway.
Journal: Genomics - Volume 89, Issue 6, June 2007, Pages 697–707